Determination of covalently bound hypusine and deoxyhypusine to protein using submilligram of protein samples by HPLC.

T BEPPU, A SHIRAHATA, K SAMEJIMA

文献索引：Beppu, Takanobu; Shirahata, Akira; Samejima, Keijiro Biological and Pharmaceutical Bulletin, 1996 , vol. 19, # 1 p. 1 - 5

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被引用次数: 5

摘要

A sensitive and reliable method for the determination of hypusine and deoxyhypusine in eIF- 5A protein, an initiation factor of protein synthesis, was developed. An advantage of this method is the use of N ε-(5-aminopentyl) lysine, an analogue of deoxyhypusine, as an internal standard. The application made it possible to determine hypusine in less than a mg of protein samples from cultured HTC cells and rat organs. After acid hydrolysis of protein ...