A sensitive and reliable method for the determination of hypusine and deoxyhypusine in eIF- 5A protein, an initiation factor of protein synthesis, was developed. An advantage of this method is the use of N ε-(5-aminopentyl) lysine, an analogue of deoxyhypusine, as an internal standard. The application made it possible to determine hypusine in less than a mg of protein samples from cultured HTC cells and rat organs. After acid hydrolysis of protein ...
