A novel ion-exclusion chromatography–mass spectrometry method to measure concentrations and cycling rates of carbohydrates and amino sugars in freshwaters
• Novel HPLC–MS method for carbohydrate and amino sugar analysis in freshwaters. • Rapid and efficient determination without derivatization or sample pre-concentration. • Implementation of stable isotope tracers to study compound biogeochemical cycling. • Glucose and N-acetyl-glucosamine showed different in situ dynamics in Lake Zurich.