前往化源商城

Biochimica et Biophysica Acta 2014-09-01

Glycan specificity of a testis-specific lectin chaperone calmegin and effects of hydrophobic interactions.

Masafumi Sakono, Akira Seko, Yoichi Takeda, Jun-ichi Aikawa, Masakazu Hachisu, Akihiko Koizumi, Kohki Fujikawa, Yukishige Ito

文献索引:Biochim. Biophys. Acta 1840(9) , 2904-13, (2014)

全文:HTML全文

摘要

Testis-specific chaperone calmegin is required for the generation of normal spermatozoa. Calmegin is known to be a homologue of endoplasmic reticulum (ER) residing lectin chaperone calnexin. Although functional similarity between calnexin and calmegin has been predicted, detailed information concerned with substrate recognition by calmegin, such as glycan specificity, chaperone function and binding affinity, are obscure.In this study, biochemical properties of calmegin and calnexin were compared using synthetic glycans and glycosylated or non-glycosylated proteins as substrates.Whereas their amino acid sequences are quite similar to each other, a certain difference in secondary structures was indicated by circular dichroism (CD) spectrum. While both of them inhibited protein heat-aggregation to a similar extent, calnexin exhibited a higher ability to facilitate protein folding. Similarly to calnexin, calmegin preferentially recognizes monoglucosylated glycans such as Glc1Man9GlcNAc2 (G1M9). While the surface hydrophobicity of calmegin was higher than that of calnexin, calnexin showed stronger binding to substrate. We reasoned that lectin activity, in addition to hydrophobic interaction, contributes to this strong affinity between calnexin and substrate.Although their similarity in carbohydrate binding specificities is high, there seems to be some differences in the mode of substrate recognition between calmegin and calnexin.Properties of calmegin as a lectin-chaperone were revealed in comparison with calnexin.Copyright © 2014 Elsevier B.V. All rights reserved.

相关化合物

结构式 名称/CAS号 全部文献
盐酸胍 结构式 盐酸胍
CAS:50-01-1
乙腈 结构式 乙腈
CAS:75-05-8
无水氯化钙 结构式 无水氯化钙
CAS:10043-52-4
碳酸钙 结构式 碳酸钙
CAS:471-34-1
三羟甲基氨基甲烷盐酸盐 结构式 三羟甲基氨基甲烷盐酸盐
CAS:1185-53-1
二水氯化钙 结构式 二水氯化钙
CAS:10035-04-8
DL-二硫苏糖醇 结构式 DL-二硫苏糖醇
CAS:3483-12-3