前往化源商城

Simultaneous determination of the anticonvulsants, cinromide (3-bromo-n-ethylcinnamamide), 3-bromocinnamamide, and carbamazepine in plasma by high-performance liquid chromatography.

R J Perchalski, J Bruni, B J Wilder, L J Willmore

文献索引:J. Chromatogr. A. 163(2) , 187-93, (1979)

全文:HTML全文

摘要

A high-performance liquid chromatographic method is described for monitoring plasma concentrations of cinromide (3-bromo-N-ethylcinnamamide) and its de-ethylated metabolite. Carbamazepine levels can be easily measured by the same technique. The N-isopropyl analogue of cinromide is used as internal standard, and all compounds are easily separated on a reversed-phase column operated at 55 degrees with a small-diameter pre-column maintained at the same temperature. The extraction is rapid and generally applicable to plasma and urine samples that are to be analyzed by reversed-phase chromatography. Short- and long-term reproducibility studies show less than 4% relative standard deviation for replicate determinations for all drugs. Limits of quantitation are 10-20 ng/ml with an internal standard concentration of 3 micrograms/ml. Another metabolite of cinromide, 3-bromocinnamic acid, which may have some anticonvulsant effect, can be analyzed simultaneously by buffering the mobile phase and adding an ion-pairing reagent.

相关化合物

结构式 名称/CAS号 全部文献
反式-3-溴-N-乙基肉桂酰胺 结构式 反式-3-溴-N-乙基肉桂酰胺
CAS:58473-74-8
间溴肉桂酸 结构式 间溴肉桂酸
CAS:32862-97-8