Neuron 2013-10-02

Cornichons control ER export of AMPA receptors to regulate synaptic excitability.

Penelope J Brockie, Michael Jensen, Jerry E Mellem, Erica Jensen, Tokiwa Yamasaki, Rui Wang, Dane Maxfield, Colin Thacker, Frédéric Hoerndli, Patrick J Dunn, Susumu Tomita, David M Madsen, Andres V Maricq

文献索引：Neuron 80(1) , 129-42, (2013)

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摘要

The strength of synaptic communication at central synapses depends on the number of ionotropic glutamate receptors, particularly the class gated by the agonist AMPA (AMPARs). Cornichon proteins, evolutionarily conserved endoplasmic reticulum cargo adaptors, modify the properties of vertebrate AMPARs when coexpressed in heterologous cells. However, the contribution of cornichons to behavior and in vivo nervous system function has yet to be determined. Here, we take a genetic approach to these questions by studying CNI-1--the sole cornichon homolog in C. elegans. cni-1 mutants hyperreverse, a phenotype associated with increased glutamatergic synaptic transmission. Consistent with this behavior, we find larger glutamate-gated currents in cni-1 mutants with a corresponding increase in AMPAR number. Furthermore, we observe opposite phenotypes in transgenic worms that overexpress CNI-1 or vertebrate homologs. In reconstitution studies, we provide support for an evolutionarily conserved role for cornichons in regulating the export of vertebrate and invertebrate AMPARs.Copyright © 2013 Elsevier Inc. All rights reserved.