9012-37-7

• 常用中文名：酰化酶
• 常用英文名：Native Aspergillus melleus Acylase I
• CAS号：9012-37-7
• 分子式：C₃₀H₃₄Cl₂N₄O
• 分子量：537.523
• 发布时间：2018-09-25 09:11:12
• 更新时间：2026-07-02 16:35:12
• 氨基酰化酶(酰化酶I)是一种生物化学试剂,可作为生物材料或有机化合物用于生命科学相关研究。

名称

• 中文名: 氨基酰化酶
• 英文名: Aminoacylase
• 中文别名: 酰化氨基酸水解酶来源于猪肾; 酰基酶Ⅰ; L-氨基酰化酶; 酰化酶,从曲霉属所得; 酰化酶 I; 酰化酶 来源于黑曲霉; 氨基酸酰化酶; Acylase fromAspergilus sp., 酶活力≥30000u/g; AMANO 酰基转移酶; 酰化酶 来自曲霉属真菌; HRP标记的氨基酰化酶1抗体; 氨基酰化酶,从曲霉属所得; 酰基转移酶,从猪肾所得; 酰基转移酶I(猪肾); 氨基酰化酶/酰化氨基酸水解酶; ACYLASE FROM ASPERGILLUS GENUS 酰化酶,从曲霉属所得; IRDYE 800CW标记的氨基酰化酶1抗体; A801202,酰化酶; 酰化氨基酸水解酶,酰化酶,酰基转移酶 (来自猪肾); 酰化酶,L-氨基酰化酶; AP标记的氨基酰化酶1抗体; BIOTIN标记的氨基酰化酶1抗体; 酰化酶, 酶活力≥30000U/G; 酰化氨基酸水解酶
• 英文别名: ACYLASE I, IMMOBILIZED ON EUPERGIT C; ACYLASE, ASPERGILLUS MELLEUS; l-aminoacidacylase; acyclase I; Hippuricase; Dehydropeptidase II; hippuricase; N-ACYL-L-AMINO-ACID AMIDOHYDROLASE; EC 3.5.1; acylase from strtomyces iseocneus; aminoacylasei; AcylaseIExPigKidney(E.C.3.5.1.14); Aminoacylase 1; PLEXAZYM(R) AC; ACYLASE 1, FROM PIG KIDNEY; Acylase I, immobilized on Eupergit(R) C from Aspergillus sp; AMINOACYLASE; ACYLASE AMANO; N-ACYLAMINO ACID AMIDOHYDROLASE; dehydroptidaseii; ACYLASE I ADE II FROM HOG KIDNEY; Benzamidase; ACYLASE FROM PENICILLIUM SP.; Aminoacylase I; ACYLASE; L-Aminoacylase; AMINOACYLASE, IMMOBILIZED; ACYLASE I FROM HOG KIDNEY LYOPH. SALT-FR POWDER ~15 U/MG; acylaseifromhogkidney; acylase i from aspergillus melleus; aminoaciddeacylase; hippurase; Acylase 1500 Amano; acylase i from porcine kidney; alpha-n-acylaminoacidhydrolase; Acylase I; acylase from strtomyces toyocaensis; ACYLASE FROM STRTOMYCES HACHIJOENSIS, U/G; ACYLASE I FROM HOG KIDNEY, MR ~45300, U/MG; Acylase; EC: 3.5.1.14; 7-[(6,8-Dichloro-1,2,3,4-tetrahydro-9-acridinyl)amino]-N-[2-(1H-indol-3-yl)ethyl]heptanamide; EC 3.5.1.14; ACYLASE HOG KIDNEY; N-Acyl-L-amino acid amidohydrolase; ACYLASE FROM STRTOMYCES ZAOMYCETICUS, ~0.25 U/MG; ACYLASE FROM STRTOMYCES TOYOCAENSIS, ~40 U/G; ACYLASE I ADE I FROM PORCINE KIDNEY; acylase from strtomyces hachijoensis; Histozyme; ACYLASE 1; FROM HOG KIDNEY; 7-[(6,8-dichloro-1,2,3,4-tetrahydroacridin-9-yl)amino]-N-[2-(1H-indol-3-yl)ethyl]heptanamide; Amino acid deacylase; AcylaseIfromporcinekidneylyophilizedpowder; AMINOACYLASE, ASPERGILLUS MELLEUS; L-Amino acid acylase; ACYLASE I FROM HOG KIDNEY, ~30 U/MG; acylase from strtomyces chtreusis; EINECS 232-732-3; acylase from aspergillus melleus; Acylase Amano, Aminoacylase; L-AMINOACYLASE; E.C. 3.5.1.14; acylase from strtomyces zaomyceticus; ACYLASE I; ACYLASE FROM STRTOMYCES CHTREUSIS, >0.1U/MG; Acylase 001; ACYLASE: FROM HOG KIDNEY; Hippurase; benzamidase; ACYLASE FROM ASPERGILLUS MELLEUS, U/MG; MFCD00081285; histozyme; Aminoacylase, immobilized, Plexazym(R) AC; ACYLASE FROM STRTOMYCES ISEOCNEUS, ~0.15 U/MG; ACYLASE I FROM ASPERGILLUS MELLEUS, U/MG

物化性质

• 密度: 1.3±0.1 g/cm3
• 沸点: 813.3±65.0 °C at 760 mmHg
• 分子式: C₃₀H₃₄Cl₂N₄O
• 分子量: 537.523
• 闪点: 445.7±34.3 °C
• 精确质量: 536.210938
• LogP: 7.64
• 外观性状: 固体;White to Amber powder to crystalline
• 蒸汽压: 0.0±2.9 mmHg at 25°C
• 折射率: 1.668
• 储存条件:

  密封-20℃干燥保存。

• 稳定性:

  Analysis Note Protein determined by biuret.

  Unit Definition One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

  Biochem/physiol Actions The enzyme hydrolyzes a variety of N-acyl-L-amino acids and dehydropeptides containing dehydroalanyl residues. N-acyl-D-amino acids and dehydropeptides other than dehydroalanine are not hydrolyzed. The following compounds are hydrolyzed by acylase I: chloroacetyl-L-amino acids, L-leucinamide, chloroacetyldehydroalanine, glycyl-L-amino acids, and glycyldehydroalanine. Aminoacylase is widely used as a reagent to resolve amino acid racemates. The Km for acyl amino acids have been reported in the literature: Ac-L-Met (0.99 mM), Ac-L-Glu (10.2 mM), and Ac-L-Phe (5.5 mM).

  Aminoacylase from pig kidney is activated by Zn2+ and Co2+ and inhibited by SH blocking reagents, disulfide reducing reagents, N-tosyl-L-alanine,

  DL-norleucine, and N-tosyl-L-lysine chloromethylketone.

• 计算化学:

  1.疏水参数计算参考值（XlogP）:7.6

  2.氢键供体数量:3

  3.氢键受体数量:3

  4.可旋转化学键数量:11

  5.互变异构体数量:12

  6.拓扑分子极性表面积69.8

  7.重原子数量:37

  8.表面电荷:0

  9.复杂度:726

  10.同位素原子数量:0

  11.确定原子立构中心数量:0

  12.不确定原子立构中心数量:0

  13.确定化学键立构中心数量:0

  14.不确定化学键立构中心数量:0

  15.共价键单元数量:1

• 更多:

  1. 性状：浅红色或浅黄色冻干粉。

  2. 密度（g/mL,25/4℃）：未确定

  3. 相对蒸汽密度（g/mL,空气=1）：未确定

  4. 熔点（ºC）：未确定

  5. 沸点（ºC,常压）：未确定

  6. 沸点（ºC, 5.2kPa）：未确定

  7. 折射率：未确定

  8. 闪点（ºC）：未确定

  9. 比旋光度（º ）：未确定

  10. 自燃点或引燃温度（ºC）：未确定

  11. 蒸气压（kPa,25ºC）：未确定

  12. 饱和蒸气压（kPa,60ºC）：未确定

  13. 燃烧热（KJ/mol）：未确定

  14. 临界温度（ºC）：未确定

  15. 临界压力（KPa）：未确定

  16. 油水（辛醇/水）分配系数的对数值：未确定

  17. 爆炸上限（%,V/V）：未确定

  18. 爆炸下限（%,V/V）：未确定

  19. 溶解性：溶于水，最适pH值7.0-7.5(猪肾、淀粉酶)或7-8(米曲酶)。

生物活性

• 描述: 氨基酰化酶(酰化酶I)是一种生物化学试剂,可作为生物材料或有机化合物用于生命科学相关研究。
• 相关类别:
  研究领域 >> 其他
  信号通路 >> 其他 >> 其他

安全

• 符号: GHS07, GHS08
• 信号词: Danger
• 危害声明: H315-H319-H334-H335
• 警示性声明: P261-P305 + P351 + P338-P342 + P311
• 个人防护装备: Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
• 危害码 (欧洲): Xn
• 风险声明 (欧洲): 36/37/38-42
• 安全声明 (欧洲): 22-24/25
• 危险品运输编码: NONH for all modes of transport
• WGK德国: 3
• RTECS号: BF4890000

制备

以猪肾、淀粉酶或米曲霉为原料，经匀浆、离心去沉淀后，用硫酸铵分级沉淀，经水透析、离心后得粗酶液，再分别用丙酮、乙醇分级沉淀、DEAE-纤维素柱层析纯化后得产品。