9028-38-0

• 常用中文名：D-3-羟丁酸脱氢酶
• 常用英文名：3-Hydroxybutyrate dehydrogenase
• CAS号：9028-38-0
• 分子式：C₃₁H₃₈ClN₅O
• 分子量：532.119
• 相关类别： 食品添加剂 酶制剂
• 发布时间：2018-06-11 12:06:53
• 更新时间：2026-07-07 12:46:10

• 二、用途

  用于生化研究。

名称

• 中文名: 3-羟基丁酸酯脱氢酶
• 英文名: Dehydrogenase, 3-Hydroxybutyrate
• 中文别名: Β-羟丁酸脱氢酶/D-3-羟丁酸脱氢酶/3-羟基丁酸脱氢酶/Β-羟基丁酸脱氢酶/-羟基丁酸酯脱氢酶/Β-HYDROXYBUTYRATE DEHYDROGENASE; 3-羟丁酸脱氢酶; Β-羟丁酸脱氢酶 来源于球形红假单胞菌; D-3-羟基丁酸酯,脱氢酶; Β-羟丁酸脱氢酶/D-3-羟丁酸脱氢酶/3-羟基丁酸脱氢酶/Β-羟基丁酸脱氢酶/D-3-羟基丁酸酯脱氢酶; 3-羟基丁酸脱氢酶; (R)-3-羟基丁酸:NAD+ 氧化还原酶; Β-羟丁酸脱氢酶(3-HBDH); 3-羟基丁酸脱氢酶(红假单胞球菌); Β-羟基丁酸脱氢酶; Β-羟丁酸脱氢酶/BR; β-羟丁酸脱氢酶; Β-羟丁酸脱氢酶/D-3-羟丁酸脱氢酶/3-羟基丁酸脱氢酶/Β-羟基丁酸脱氢酶/-羟基丁酸酯脱氢酶; Β-羟丁酸脱氢酶,3-羟丁酸脱氢酶; Β-羟丁酸脱氢酶; Β-羟丁酸脱氢酶 来源于勒氏假单胞菌; Β-羟丁酸脱氢酶, 400 单位/毫克; 3-羟丁酸脱氢酶(HBDH); 羟丁酸脱氢酶; D-3-羟丁酸脱氢酶(HBDH)
• 英文别名: EC 1.1.1.30 TYPE V; beta-hydroxybutyrate dehydrogenase type v; MFCD00130633; 3-Hydroeybutyrate Dehydrogenase（HBDH）; β-Hydroxybutyrate Dehydrogenase; N-{3-[{3-[(6-Chloro-1,2,3,4-tetrahydro-9-acridinyl)amino]propyl}(methyl)amino]propyl}-3-(1H-indol-3-yl)propanamide; Native Rhodopseudomonas sphaeroides β-Hydroxybutyrate Dehydrogenase; ec 1.1.1.30; ec: 1.1.1.30; 3-Hydroxybutyrate Dehydrogenase “Amano” 5; beta-Hydroxybutyrate Dehydrogenase Activity Microplate Assay Kit; d-3-hydroxybutyrate: nad oxidoreductase; B-HYDROXYBUTYRATE DEHYDROGENASE TYPE IV FROM PSEUDO; (R)-3-Hydroxybutanoate: NAD+ oxidoreductase, 3-HBDH, 3-Hydroxybutyrate Dehydrogenase solution from Rhodopseudomonas sphaeroides; beta-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides; D-3-hydroxybutyrate dehydrogenase from Microorganism; beta-hydroxybutyrate dehydrogenase; C:C&B15; β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei; D-3-HYDROXYBUTYRATE: NAD OXIDOREDUCTASE; D-3-HYDROXYBUTYRATE DEHYDROGENASE; D-3-Hydroxybutyrate dehydrogenase（HBDH）; Prokyotic 3-Hydroxybutyrate dehydrogenase, Recombinant; beta-Hydroxybutyrate Dehydrogenase Rhodopseudomonas sphaeroides; ec 1.1.1.30 type v; (R)-3-HYDROXYBUTANOATE: NAD+ OXIDOREDUCTASE; Native Pseudomonas sp. D-3-hydroxybutyrate dehydrogenase; β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei,(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH; D-3-Hydroxybutyrate Dehydrogenase,from Pseudomonas; N-{3-[{3-[(6-chloro-1,2,3,4-tetrahydroacridin-9-yl)amino]propyl}(methyl)amino]propyl}-3-(1H-indol-3-yl)propanamide; B-HYDROXYBUTYRATE DEHYDROGENASE TYPE V; d-3-hydroxybutyrate dehydrogenase; Native Alcaligenes faecalis 3-Hydroxybutyrate Dehydrogenase; beta-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei; Dehydrogenase, 3-hydroxybutyrate; BETA-HYDROXYBUTYRATE DEHYDROGENASE; EC: 1.1.1.30; [R]-3-HYDROXYBUTANOATE: NAD+ OXIDOREDUCTASE TYPE V; β-HBDH; β-hydroxybutyrate dehydrogenase from rhodopseudomonas sphaeroides; 3-Hydroeybutyrate Dehydrogenase; EC 1.1.1.30; 3-hbdh; D-3-Hydroxybutyrate Dehydrogenase (3-HBDH) ex. Pseudomonas Sp., 100U/mg; B-HYDROXYBUTYRATE DEHYDROGENASE*TYPE III; 3-Hydroxybutyrate Dehydrogenase solution from Rhodopseudomonas sphaeroides; B-HYDROXYBUTYRATE DEHYDROGENASE; B-HYDROXYBUTYRATE DEHYDROGENASE TYPE II; Dehydrogese,3-hydroxybutyrate; β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides,(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH; BETA-HYDROXYBUTYRATE DEHYDROGENASE TYPE V; Native Pseudomonas lemoignei β-Hydroxybutyrate Dehydrogenase; D-3-HYDROXYBUTYRATE DEHYDROGENASE //BP; β-Hydroxybutyrate Dehydrogenase [>=400 units/mg]; 3-HBDH; D-Hydroxybutyrate Dehydrogenase; 3-hydroxybutyrate dehydrogenase F.rhodo-pseudomon.spheroid; 3-HYDROXYBUTYRATE DEHYDROGENASE

物化性质

• 密度: 1.2±0.1 g/cm3
• 沸点: 806.8±65.0 °C at 760 mmHg
• 分子式: C₃₁H₃₈ClN₅O
• 分子量: 532.119
• 闪点: 441.7±34.3 °C
• 精确质量: 531.276489
• LogP: 5.89
• 外观性状: lyophilized powder
• 蒸汽压: 0.0±2.9 mmHg at 25°C
• 折射率: 1.663
• 储存条件:

  一、贮存

  应充氩气密封于0℃以下（粉末）和4℃（悬浮液）保存。

• 稳定性:

  Application Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

  Biochem/physiol Actions In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

  Unit Definition One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

  Physical form Lyophilized powder containing sucrose and Tris buffer salts

• 计算化学:

  1.疏水参数计算参考值（XlogP）:6.1

  2.氢键供体数量:3

  3.氢键受体数量:4

  4.可旋转化学键数量:12

  5.互变异构体数量:12

  6.拓扑分子极性表面积73

  7.重原子数量:38

  8.表面电荷:0

  9.复杂度:739

  10.同位素原子数量:0

  11.确定原子立构中心数量:0

  12.不确定原子立构中心数量:0

  13.确定化学键立构中心数量:0

  14.不确定化学键立构中心数量:0

  15.共价键单元数量:1

• 更多:

  1. 性状：冻干粉末。

  2. 密度（g/mL,25℃）：未确定

  3. 相对蒸汽密度（g/mL,空气=1）：未确定

  4. 熔点（ºC）：未确定

  5. 沸点（ºC,常压）：未确定

  6. 沸点（ºC,5.2kPa）：未确定

  7. 折射率：未确定

  8. 闪点（ºC）：未确定

  9. 比旋光度（º）：未确定

  10. 自燃点或引燃温度（ºC）：未确定

  11. 蒸气压（kPa,20ºC）：未确定

  12. 饱和蒸气压（kPa,60ºC）：未确定

  13. 燃烧热（KJ/mol）：未确定

  14. 临界温度（ºC）：未确定

  15. 临界压力（KPa）：未确定

  16. 油水（辛醇/水）分配系数的对数值：未确定

  17. 爆炸上限（%,V/V）：未确定

  18. 爆炸下限（%,V/V）：未确定

  19. 溶解性：溶于水

生物活性

• 描述: 3-羟基丁酸脱氢酶(3-HBDH)是一种线粒体酶,常用于生化研究。3-羟基丁酸脱氢酶参与酮体的合成和降解以及丁酸盐代谢。3-羟基丁酸脱氢酶催化(R)-3-羟基丁酸酯转化为乙酰乙酸盐[1]。
• 相关类别:
  研究领域 >> 其他
• 参考文献:

  [1]. Watson HR, Lindsay DB. 3-hydroxybutyrate dehydrogenase in tissues from normal and ketonaemic sheep. Biochem J. 1972 Jun;128(1):53-7.

安全

• 个人防护装备: Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)
• 危害码 (欧洲): Xi
• 风险声明 (欧洲): 36/37/38
• 安全声明 (欧洲): 26-36-24/25
• 危险品运输编码: NONH for all modes of transport
• WGK德国: 3
• 海关编码: 35079090