115534-33-3

• 常用中文名：TMA-DPH
• 常用英文名：TMA-DPH
• CAS号：115534-33-3
• 分子式：C₂₈H₃₁NO₃S
• 分子量：461.61600
• 相关类别： 染料试剂
• 发布时间：2017-05-13 22:13:04
• 更新时间：2024-01-06 10:06:56
• TMA-DPH 是一种疏水性荧光膜探针。

名称

• 中文名: TMA-DPH
• 英文名: N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium p-toluenesulfonate
• 英文别名: TMA-DPH [N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium,p-toluenesulfonate]; 4-methylbenzenesulfonate,trimethyl-[4-[(1E,3E,5E)-6-phenylhexa-1,3,5-trienyl]phenyl]azanium; TMA-DPH

物化性质

• 熔点: 237ºC (dec.)(lit.)
• 分子式: C₂₈H₃₁NO₃S
• 分子量: 461.61600
• 精确质量: 461.20200
• PSA: 65.58000
• LogP: 7.14600
• 储存条件: -20°C，密闭，干燥

生物活性

• 描述: TMA-DPH 是一种疏水性荧光膜探针。
• 相关类别:
  信号通路 >> 其他 >> 其他
  染料试剂
  研究领域 >> 其他
• 体外研究: 与细胞接触时,TMA-DPH瞬间掺入质膜中。 TMA-DPH浓缩在溶酶体和高度酸性的晚期内体中[1]。 TMA-DPH荧光寿命保持恒定,6.2±0.2 ns,低于相同的2μM临界浓度,但随着浓度的增加而显着降低。然而,这种下降速度减慢到5μM以上。 TMA-DPH荧光各向异性显示出与荧光寿命类似的演变。它首先是常数,0.283±0.003,再次直到2μM,然后在5μM时迅速下降至0.270±0.003,继续下降,但浓度较高时速率较低[2]。
• 细胞实验: 使2mL DM10F中的L929细胞粘附在显微镜载玻片上。为了观察质膜标记，将细胞在室温下用TMA-DPH 2×10-6M的PBS溶液或DM10F从4×10-3M的二甲基甲酰胺储备液中培养短时间（10秒）。然后将未洗涤的载玻片转移到显微镜中并观察。对于内化膜的标记，将细胞在37℃的载玻片中，在DM10F中用TMA-DPH 2×10-6M孵育所需的时间，然后通过在PBS中轻轻摇动载玻片几秒钟来洗涤。 [1]。
• 参考文献:

  [1]. Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125(4):783-94.

  [2]. Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66.

MSDS

Section1. IDENTIFICATION OF THE SUBSTANCE/MIXTURE Product identifiers Product name: N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1- yl)phenylammonium p-toluenesulfonate CAS-No.: 115534-33-3 Relevant identified uses of the substance or mixture and uses advised against Identified uses: Laboratory chemicals, Manufacture of substances Section2. HAZARDS IDENTIFICATION Classification of the substance or mixture Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. Label elements The product does not need to be labelled in accordance with EC directives or respective national laws. Caution - substance not yet tested completely. Other hazards - none Section3. COMPOSITION/INFORMATION ON INGREDIENTS Substances Synonyms: TMA-DPH 4′-(Trimethylammonio)diphenylhexatriene p-toluenesulfonate 1,6-Diphenyl-1,3,5-hexatriene-4′-trimethylammonium tosylate 1-(4-Trimethylammoniophenyl)-6-phenyl-1,3,5-hexatriene p- toluenesulfonate Formula: C28H31NO3S Molecular Weight: 461,62 g/mol Section4. FIRST AID MEASURES Description of first aid measures If inhaled If breathed in, move person into fresh air. If not breathing, give artificial respiration. In case of skin contact Wash off with soap and plenty of water. In case of eye contact Flush eyes with water as a precaution. If swallowed Never give anything by mouth to an unconscious person. Rinse mouth with water. Most important symptoms and effects, both acute and delayed Indication of any immediate medical attention and special treatment needed no data available Section5. FIREFIGHTING MEASURES Extinguishing media Suitable extinguishing media Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Special hazards arising from the substance or mixture Carbon oxides, nitrogen oxides (NOx), Sulphur oxides Advice for firefighters Wear self contained breathing apparatus for fire fighting if necessary. Further information no data available Section6. ACCIDENTAL RELEASE MEASURES Personal precautions, protective equipment and emergency procedures Avoid dust formation. Avoid breathing vapors, mist or gas. Environmental precautions Do not let product enter drains. Methods and materials for containment and cleaning up Sweep up and shovel. Keep in suitable, closed containers for disposal. Reference to other sections For disposal see section 13. Section7. HANDLING AND STORAGE Precautions for safe handling Provide appropriate exhaust ventilation at places where dust is formed. Conditions for safe storage, including any incompatibilities Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Recommended storage temperature: -20 °C Light sensitive. Specific end uses no data available Section8. EXPOSURE CONTROLS/PERSONAL PROTECTION Control parameters Components with workplace control parameters Exposure controls Appropriate engineering controls General industrial hygiene practice. Personal protective equipment Eye/face protection Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU). Skin protection Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and the standard EN 374 derived from it. Body Protection Choose body protection in relation to its type, to the concentration and amount of dangerous substances, and to the specific work-place., The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace. Respiratory protection Respiratory protection is not required. Where protection from nuisance levels of dusts are desired, use type N95 (US) or type P1 (EN 143) dust masks. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU). Section9. PHYSICAL AND CHEMICAL PROPERTIES Information on basic physical and chemical properties a) AppearanceForm: solid b) Odourno data available c) Odour Thresholdno data available d) pHno data available e) Melting point/freezingMelting point/range: 237 °C - lit. point f) Initial boiling point and no data available boiling range g) Flash pointno data available h) Evaporation rateno data available i) Flammability (solid, gas) no data available j) Upper/lowerno data available flammability or explosive limits k) Vapour pressureno data available l) Vapour densityno data available m) Relative densityno data available n) Water solubilityinsoluble o) Partition coefficient: n- no data available octanol/water p) Autoignitionno data available temperature q) Decompositionno data available temperature r) Viscosityno data available s) Explosive propertiesno data available t) Oxidizing propertiesno data available Other safety information no data available Section10. STABILITY AND REACTIVITY Reactivity no data available Chemical stability no data available Possibility of hazardous reactions no data available Conditions to avoid no data available Incompatible materials Strong oxidizing agents Hazardous decomposition products Section11. TOXICOLOGICAL INFORMATION Information on toxicological effects Acute toxicity no data available Skin corrosion/irritation no data available Serious eye damage/eye irritation no data available Respiratory or skin sensitization no data available Germ cell mutagenicity no data available Carcinogenicity IARC:No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. Reproductive toxicity no data available Specific target organ toxicity - single exposure no data available Specific target organ toxicity - repeated exposure no data available Aspiration hazard no data available Potential health effects InhalationMay be harmful if inhaled. May cause respiratory tract irritation. Ingestion May be harmful if swallowed. SkinMay be harmful if absorbed through skin. May cause skin irritation. EyesMay cause eye irritation. Additional Information RTECS: Not available Section12. ECOLOGICAL INFORMATION Toxicity no data available Persistence and degradability no data available Bioaccumulative potential no data available Mobility in soil no data available Results of PBT and vPvB assessment no data available Other adverse effects no data available Section13. DISPOSAL CONSIDERATIONS Waste treatment methods Product Offer surplus and non-recyclable solutions to a licensed disposal company. Contaminated packaging Dispose of as unused product. Section14. TRANSPORT INFORMATION UN number ADR/RID: -IMDG: -IATA: - UN proper shipping name ADR/RID: Not dangerous goods IMDG: Not dangerous goods IATA:Not dangerous goods Transport hazard class(es) ADR/RID: -IMDG: -IATA: - Packaging group ADR/RID: -IMDG: -IATA: - Environmental hazards ADR/RID: noIMDG Marine pollutant: noIATA: no Special precautions for user no data available SECTION 15 - REGULATORY INFORMATION N/A SECTION 16 - ADDITIONAL INFORMATION N/A

安全

• 个人防护装备: Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
• 危害码 (欧洲): T: Toxic;
• 风险声明 (欧洲): R61
• 安全声明 (欧洲): 53-45
• 危险品运输编码: NONH for all modes of transport