4-(4-(二甲基氨基)苯乙烯)-N-甲基碘化吡啶

• 常用名: 4-(4-(二甲基氨基)苯乙烯)-N-甲基碘化吡啶
• 英文名: Pyridinium,4-[2-[4-(dimethylamino)phenyl]ethenyl]-1-methyl-, iodide (1:1)
• CAS号: 959-81-9
• 分子量: 366.24000
• 密度: N/A
• 沸点: N/A
• 分子式: C₁₆H₁₉IN₂
• 熔点: N/A
• 闪点: N/A

用途

4-Di-1-ASP是一种苯乙烯染料,用于染色活脑组织中的胶质瘤细胞,以分析细胞结构、活性、增殖和内吞、胞质分裂和吞噬,以及观察活细胞中的线粒体结构。4-Di-1-ASP在显微镜下成像时发出绿色荧光(λex/λem=475/606 nm)[1][2]。

4-(4-(二甲基氨基)苯乙烯)-N-甲基碘化吡啶名称

• 中文名: 4-(4-(二甲基氨基)苯乙烯)-N-甲基碘化吡啶
• 英文名: 4-(4-(dimethylamino)styryl)-n-methylpyridinium iodide

4-(4-(二甲基氨基)苯乙烯)-N-甲基碘化吡啶生物活性

• 描述: 4-Di-1-ASP是一种苯乙烯染料,用于染色活脑组织中的胶质瘤细胞,以分析细胞结构、活性、增殖和内吞、胞质分裂和吞噬,以及观察活细胞中的线粒体结构。4-Di-1-ASP在显微镜下成像时发出绿色荧光(λex/λem=475/606 nm)[1][2]。
• 相关类别:
  研究领域 >> 癌症
  信号通路 >> 其他 >> 其他
• 体外研究: 指南(以下是我们推荐的方案。该方案仅提供指南,应根据您的具体需要进行修改)[1]。1.将染料溶解在水中,配置浓度为2 mM的母液。2.将制备好的细胞切片置于35°C的培养箱中,将制备的染料溶液添加到最终浓度为1µM,并与染色剂一起培养10分钟。3.将制备完成的染色细胞切片放在显微镜载玻片上,用盖玻片盖住,用荧光共焦显微镜观察,最大激发波长为475nm,最大发射波长为606nm。
• 参考文献:

  [1]. Lilia Y Kucheryavykh, et al. Visualization of implanted GL261 glioma cells in living mouse brain slices using fluorescent 4-(4-(dimethylamino)-styryl)-N-methylpyridinium iodide (ASP+). Biotechniques. 2012 Nov;53(5):305-9.

  [2]. Jensen J H Wong, et al. Simultaneous High-Throughput Conformational and Colloidal Stability Screening Using a Fluorescent Molecular Rotor Dye, 4-(4-(Dimethylamino)styryl)-N-Methylpyridinium Iodide (DASPMI). J Biomol Screen. 2016 Sep;21(8):842-50.

4-(4-(二甲基氨基)苯乙烯)-N-甲基碘化吡啶物理化学性质

• 分子式: C₁₆H₁₉IN₂
• 分子量: 366.24000
• 精确质量: 366.05900
• PSA: 7.12000
• InChIKey: UJNFDSOJKNOBIA-UHFFFAOYSA-M
• SMILES: CN(C)c1ccc(C=Cc2cc[n+](C)cc2)cc1.[I-]

4-(4-(二甲基氨基)苯乙烯)-N-甲基碘化吡啶靶点实验

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实验名称：Fluorescence polarization to screen for inhibitor that competite the binding of FadD2... 来源：Broad Institute 靶标：FATTY-ACID-CoA LIGASE FADD28 (FATTY-ACID-CoA SYNTHETASE) External Id：2147-01_Inhibitor_SinglePoint_HTS_Activity

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4-(4-(二甲基氨基)苯乙烯)-N-甲基碘化吡啶英文别名

• W-(N'-METHYLPYRIDYL-4')-4-DIMETHYLAMINOSTYRENE IODIDE
• 4-{(E)-2-[4-(dimethylamino)phenyl]vinyl}-1-methylpyridinium iodide
• DASPMI
• 4'-DIMETHYLAMINO-N-METHYL-4-STILBAZOLIUM IODIDE
• 4-N,N-dimethylamino-4'-N'-methylstilbazolium trifluoromethanesulfonate
• 4-DI-1-ASP IODIDE
• 4-[P-(DIMETHYLAMINO)STYRYL]-1-METHYLPYRIDINIUM IODIDE
• 1-METHYL-4-P-DIMETHYLAMINO-STYRYL-PYRIDINIUM-IODIDE
• 4-DI-1-ASP
• trans-4-[4'-(N,N-dimethylamino)styryl]-N-methylpyridinium iodide
• [(E)-N-methyl-4-(2-(4-(N,N-dimethyl)phenyl)ethenyl)pyridinium] iodide
• 4-[4-(DIMETHYLAMINO)STYRYL]-1-METHYLPYRIDINIUM IODIDE
• 1-methyl-4-{2-[4-(dimethylamino)phenyl]ethenyl}pyridinium iodide