Design and synthesis of bis-biotin-containing reagents for applications utilizing monoclonal antibody-based pretargeting systems with streptavidin mutants

…, Y Lin, SM Wilbur, F Buchegger, A Pantelias…

Index: Wilbur, D. Scott; Park, Steven I.; Chyan, Ming-Kuan; Wan, Feng; Hamlin, Donald K.; Shenoi, Jaideep; Lin, Yukang; Wilbur, Shani M.; Buchegger, Franz; Pantelias, Anastasia; Pagel, John M.; Press, Oliver W. Bioconjugate Chemistry, 2010 , vol. 21, # 7 p. 1225 - 1238

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Citation Number: 8

Abstract

Previous studies have shown that pretargeting protocols, using cancer-targeting fusion proteins, composed of 4 anti-CD20 single chain Fv (scFv) fragments and streptavidin (scFv4- SAv), followed by a biotinylated dendrimeric N-acetyl-galactosamine blood clearing agent (CA), 1, then a radiolabeled DOTA-biotin derivative (a monobiotin), 3a, can provide effective therapy for lymphoma xenografts in mouse models. A shortcoming in this pretargeting ...