... Tetrahedron: Asymmetry. Volume 12, Issue 10, 22 June 2001, Pages 1431–1434. ... flask containing 20 mL of sterile medium consisting of (per 1000 mL) peptone (10 g) and beef extract (5 g). The culture flask was incubated at 37°C in an orbital shaker at 200 rpm for 12 h. A portion ...