Post-proline endopeptidase. Partial purification and characterization of the enzyme from pig kidneys

K Hauzer, L Servítová, T Barth…

Index: Hauzer, Karel; Servitova, Linda; Barth, Tomislav; Jost, Karel Collection of Czechoslovak Chemical Communications, 1982 , vol. 47, # 4 p. 1139 - 1148

Full Text: HTML

Citation Number: 10

Abstract

Abstract Post-proline endopeptidase was isolated from pig kidneys and partially purified. The procedure consisted of fractionation with ammonium sulphate, ion exchange chromatography on DEAE-Sephadex A-50, gel filtration on Sephadex G-200 and rechromatography on DEAE-Sephadex A-50. The preparation had 55 times higher specific activity than the crude extract and did not contain any contaminating enzymic activities. ...