Tetrahedron letters

Fast cleavage and deprotection of oligonucleotides

MP Reddy, NB Hanna, F Farooqui

Index: Reddy, M. P.; Hanna, N. B.; Farooqui, Firdous Tetrahedron Letters, 1994 , vol. 35, # 25 p. 4311 - 4314

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Citation Number: 126

Abstract

Abstract We have developed methylamine/ammonia as a fast cleavage and deprotection reagent which effects complete cleavage of oligonucleotides from the solid support in 5 min at room temperature and complete deprotection in 5 min at 65 C. The problem of transamination side product formation, faced with the commonly used dC bz (10.0% side product) upon treatment with methylamine/ammonia, has been successfully solved by the ...