p-Aminophenyl β-cellobioside (PAPC) is shown to be an effective affinity ligand for the chromatographic fractionation of cellobiohydrolases (CBHs). A crude cellulase preparation from the filamentous fungus Trichoderma reesei served as a representative source of enzymes for this study. Prior to chromatography, PAPC was tethered via its amino functional group to N-hydroxysuccinimide-activated agarose. The resulting affinity matrix specifically ...
[Muellegger, Johannes; Chen, Hong Ming; Warren, R. Antony J.; Withers, Stephen G. Angewandte Chemie - International Edition, 2006 , vol. 45, # 16 p. 2585 - 2588]