4-Nitrophenyl β-D-Cellobioside

Modify Date: 2024-01-10 17:03:33

4-Nitrophenyl β-D-Cellobioside Structure
4-Nitrophenyl β-D-Cellobioside structure
Common Name 4-Nitrophenyl β-D-Cellobioside
CAS Number 3482-57-3 Molecular Weight 463.39000
Density 1.7 g/cm3 Boiling Point 795.6ºC at 760 mmHg
Molecular Formula C18H25NO13 Melting Point 249-250ºC
MSDS Chinese USA Flash Point 435ºC

 Use of 4-Nitrophenyl β-D-Cellobioside


4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP)[1][2].

 Names

Name 4-Nitrophenyl β-D-cellobioside
Synonym More Synonyms

 4-Nitrophenyl β-D-Cellobioside Biological Activity

Description 4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP)[1][2].
Related Catalog
References

[1]. Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371(2):146-53.  

[2]. Yukihiko Hayashi, et al. Transcellobiosylation Reactions Catalyzed by Different Exoglucanase Components of a T richoderma viride Cellulase in Aqueous Organic Solvent. Biocatal Biotransfor, 2003, 21(1): 25-31.

 Chemical & Physical Properties

Density 1.7 g/cm3
Boiling Point 795.6ºC at 760 mmHg
Melting Point 249-250ºC
Molecular Formula C18H25NO13
Molecular Weight 463.39000
Flash Point 435ºC
Exact Mass 463.13300
PSA 224.35000
Index of Refraction 1.67
Storage condition 2-8°C
Water Solubility methanol: water (2:3): 50 mg/mL, clear, faintly yellow

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases S24/25
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 29400090

 Synthetic Route

 Articles25

More Articles
Mechanistic studies of active site mutants of Thermomonospora fusca endocellulase E2.

Biochemistry 38(30) , 9746-51, (1999)

Endocellulase E2 from the thermophilic bacterium Thermomonospora fusca is a member of glycosyl-hydrolase family 6 and is active from pH 4 to 10. Enzymes in this family hydrolyze beta-1,4-glycosidic bo...

Structural changes of cellobiohydrolase I (1,4-beta-D-glucan-cellobiohydrolase I, CBHI) and PNPC (p-nitrophenyl-beta-D-cellobioside) during the binding process.

Sci. China,. C, Life Sci. 51(5) , 459-69, (2008)

Conformational changes to 1,4-beta-D-glucan cellobiohydrolase I (CBHI) in response to its binding with p-nitrophenyl beta-D-cellobioside (PNPC) were analyzed by second-derivative fluorescence spectrom...

The competitive inhibition of Trichoderma reesei C30 cellobiohydrolase I by guanidine hydrochloride.

FEBS Lett. 270(1-2) , 143-6, (1990)

The p-nitrophenylcellobiosidase (PNPCase) activity of Trichoderma reesei cellobiohydrolase I (CBH I) was competitively inhibited by concentrations of guanidine hydrochloride (Gdn HCl) that did not aff...

 Synonyms

4-NITROPHENYL-β-D-CELLOBIOSIDE
4-Nitrophenyl β-D-Cellobioside
4-NITROPHENYL-BETA-D-CELLOBIOSIDE
4-Nitrophenyl beta-D-Cellobioside
MFCD00069845