4-Nitrophenyl β-D-Cellobioside

Modify Date: 2025-08-26 10:00:40

4-Nitrophenyl β-D-Cellobioside structure	Common Name	4-Nitrophenyl β-D-Cellobioside
	CAS Number	3482-57-3	Molecular Weight	463.39000
	Density	1.7 g/cm3	Boiling Point	795.6ºC at 760 mmHg
	Molecular Formula	C₁₈H₂₅NO₁₃	Melting Point	249-250ºC
	MSDS	Chinese USA	Flash Point	435ºC

Use of 4-Nitrophenyl β-D-Cellobioside

4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP)[1][2].

Name	4-Nitrophenyl β-D-cellobioside
Synonym	More Synonyms

Description	4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP)[1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
References	[1]. Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371(2):146-53. [2]. Yukihiko Hayashi, et al. Transcellobiosylation Reactions Catalyzed by Different Exoglucanase Components of a T richoderma viride Cellulase in Aqueous Organic Solvent. Biocatal Biotransfor, 2003, 21(1): 25-31.

Description

Related Catalog

Research Areas >> Others

Signaling Pathways >> Others >> Others

References

[1]. Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371(2):146-53.

[2]. Yukihiko Hayashi, et al. Transcellobiosylation Reactions Catalyzed by Different Exoglucanase Components of a T richoderma viride Cellulase in Aqueous Organic Solvent. Biocatal Biotransfor, 2003, 21(1): 25-31.

Density	1.7 g/cm3
Boiling Point	795.6ºC at 760 mmHg
Melting Point	249-250ºC
Molecular Formula	C₁₈H₂₅NO₁₃
Molecular Weight	463.39000
Flash Point	435ºC
Exact Mass	463.13300
PSA	224.35000
Index of Refraction	1.67
Storage condition	2-8°C
Water Solubility	methanol: water (2:3): 50 mg/mL, clear, faintly yellow

4-Nitrophenyl β-D-Cellobioside MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases	S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3
HS Code	29400090

Precursor 9
CAS#:69948-03-4 p-Nitrophenyl-D... CAS#:2106-10-7 α-D-Glucopyrano... CAS#:2492-87-7 PNPG CAS#:5328-50-7 Altropyranose, ...
CAS#:133-99-3 Cellobiose CAS#:83-87-4 beta-d-glucose ... CAS#:5987-78-0 4'-nitrophenyl ... CAS#:100-02-7 4-Nitrophenol
CAS#:604-69-3 β-D-Glucose pen...
DownStream 10
CAS#:106927-48-4 p-Nitrophenyl β... CAS#:129411-62-7 p-nitrophenyl b... CAS#:100-02-7 4-Nitrophenol CAS#:133-99-3 Cellobiose
CAS#:3767-28-0 4-nitrophenyl α... CAS#:492-62-6 dextrose CAS#:619-08-9 2-Chloro-4-nitr... CAS#:6401-81-6 α-D-Glcp-(1->4)...
CAS#:2492-87-7 PNPG CAS#:42935-24-0 p-Aminophenyl-b...

Mechanistic studies of active site mutants of Thermomonospora fusca endocellulase E2.

Biochemistry 38(30) , 9746-51, (1999)

Endocellulase E2 from the thermophilic bacterium Thermomonospora fusca is a member of glycosyl-hydrolase family 6 and is active from pH 4 to 10. Enzymes in this family hydrolyze beta-1,4-glycosidic bo...

Structural changes of cellobiohydrolase I (1,4-beta-D-glucan-cellobiohydrolase I, CBHI) and PNPC (p-nitrophenyl-beta-D-cellobioside) during the binding process.

Sci. China,. C, Life Sci. 51(5) , 459-69, (2008)

Conformational changes to 1,4-beta-D-glucan cellobiohydrolase I (CBHI) in response to its binding with p-nitrophenyl beta-D-cellobioside (PNPC) were analyzed by second-derivative fluorescence spectrom...

The competitive inhibition of Trichoderma reesei C30 cellobiohydrolase I by guanidine hydrochloride.

FEBS Lett. 270(1-2) , 143-6, (1990)

The p-nitrophenylcellobiosidase (PNPCase) activity of Trichoderma reesei cellobiohydrolase I (CBH I) was competitively inhibited by concentrations of guanidine hydrochloride (Gdn HCl) that did not aff...

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MFCD00069845