Functional and mechanistic analyses of biomimetic aminoacyl transfer reactions in de novo designed coiled coil peptides via rational active site engineering

…, DA Weinberger, ZZ Huang, KM Wilcoxen…

Index: Wilcoxen, Keith M.; Leman, Luke J.; Weinberger, Dana A.; Huang, Zheng-Zheng; Ghadiri, M. Reza Journal of the American Chemical Society, 2007 , vol. 129, # 4 p. 748 - 749

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Citation Number: 18

Abstract

Ribosomes and nonribosomal peptide synthetases (NRPSs) carry out instructed peptide synthesis through a series of directed intermodular aminoacyl transfer reactions. We recently reported the design of coiled-coil assemblies that could functionally mimic the elementary aminoacyl loading and intermodular aminoacyl transfer steps of NRPSs. These peptides were designed initially to accelerate aminoacyl transfer mainly through catalysis by ...