Efficient Access to Nonhydrolyzable Initiator tRNA Based on the Synthesis of 3′??Azido??3′??Deoxyadenosine RNA

…, D Graber, H Moroder, AS Geiermann…

Index: Steger, Jessica; Graber, Dagmar; Moroder, Holger; Geiermann, Anna-Skrollan; Aigner, Michaela; Micura, Ronald Angewandte Chemie - International Edition, 2010 , vol. 49, # 41 p. 7470 - 7472

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Citation Number: 24

Abstract

3'-Aminoacyl-tRNA conjugates with a hydrolysis-resistant amide linkage instead of the natural ester represent valuable substrates for biochemical studies of ribosomal processes. Access to such conjugates is currently a serious bottleneck for the investigation and functional characterization of pre-and post-peptidyl-transfer states,[1] of the tRNA hybrid states,[2] of translation initiation [2] and termination,[3] as well as of phenomena like ...