Avidin-biotin affinity columns. General methods for attaching biotin to peptides and proteins

K Hofmann, FM Finn, Y Kiso

Index: Hofmann,K. et al. Journal of the American Chemical Society, 1978 , vol. 100, # 11 p. 3585 - 3590

Full Text: HTML

Citation Number: 71

Abstract

Abstract: The AB catch principle is a novel technique for the preparation of affinity columns that is based on the high affinity of the B-complex vitamin biotin for the egg-white protein avidin. One advantage of the technique involves the attachment of biotin to biologically active molecules in a targeted manner that does not impair biological activity. This communication relates synthetic routes by biocytinamide and to Ne-p- ...