Carbohydrate Research 2015-03-02

Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.

Evelina L Zdorovenko, Alexander S Shashkov, Marina V Zhurina, Vladimir K Plakunov, Yuriy A Knirel

Index: Carbohydr. Res. 404 , 93-7, (2015)

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Abstract

O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.Copyright © 2014 Elsevier Ltd. All rights reserved.