Biotechnology and Applied Biochemistry 2015-01-01

Use of sulfur-oxidizing bacteria as recognition elements in hydrogen sulfide biosensing system.

Behdokht Janfada, Fatemeh Yazdian, Ghassem Amoabediny, Mahdi Rahaie

Index: Biotechnol. Appl. Biochem. 62 , 349-56, (2015)

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Abstract

Four sulfur-oxidizing bacteria (Thiobacillus thioparus, Acidithiobacillus thiooxidans PTCC1717, Acidithiobacillus ferrooxidans PTCC1646, and Acidithiobacillus ferrooxidans PTCC1647) were used as biorecognition elements in a hydrogen sulfide biosensing system. All the experiments were performed in 0.1 M phosphate buffer solution containing 1-20 ppm H2S with optimum pH and temperature for each species. Although H2 S was applied to the biosensing system, the dissolved O2 content decreased. Dissolved O2 consumed by cells in both free and immobilized forms was measured using a dissolved oxygen sensor. Free bacterial cells exhibit fast response (<200 Sec). Immobilization of the cells on polyvinyl alcohol was optimized using an analytical software. Immobilized A. ferrooxidans and A. thiooxidans retained more than 50% of activity after 30 days of immobilization. According to the data, A. thiooxidans and A. ferrooxidans are appropriate species for hydrogen sulfide biosensor.© 2014 International Union of Biochemistry and Molecular Biology, Inc.