Journal of chromatography. A 2014-11-28

Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide.

Abed Abdel Qader, Javier Urraca, Silje Bøen Torsetnes, Finn Tønnesen, Léon Reubsaet, Börje Sellergren

Index: J. Chromatogr. A. 1370 , 56-62, (2014)

Full Text: HTML

Abstract

Peptide imprinted polymers were developed for detection of progastrin releasing peptide (ProGRP); a low abundant blood based biomarker for small cell lung cancer. The polymers targeted the proteotypic nona-peptide sequence NLLGLIEAK and were used for selective enrichment of the proteotypic peptide prior to LCMS based quantification. Peptide imprinted polymers with the best affinity characteristics were first identified from a 96-polymer combinatorial library. The effects of functional monomers, crosslinker, porogen, and template on adsorption capacity and selectivity for NLLGLIEAK were investigated and optimized. Ultimately, a solid phase extraction method was developed for highly selective enrichment of the target peptide from tryptic digests. Copyright © 2014 Elsevier B.V. All rights reserved.