Rapid Communications in Mass Spectrometry 1994-09-01

Detection of 500-nucleotide DNA by laser desorption mass spectrometry.

K Tang, N I Taranenko, S L Allman, L Y Cháng, C H Chen

Index: Rapid Commun. Mass Spectrom. 8 , 727-730, (1994)

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Abstract

We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.