European Journal of Pharmaceutics and Biopharmaceutics 2015-06-01

Curcumin loaded PLGA-poloxamer blend nanoparticles induce cell cycle arrest in mesothelioma cells.

Laura Mayol, Carla Serri, Ciro Menale, Stefania Crispi, Maria Teresa Piccolo, Luigi Mita, Simona Giarra, Maurizio Forte, Antonina Saija, Marco Biondi, Damiano Gustavo Mita

Index: Eur. J. Pharm. Biopharm. 93 , 37-45, (2015)

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Abstract

The pharmacological potential of curcumin (CURC) is severely restricted because of its low water solubility/absorption, short half-life and poor bioavailability. To overcome these issues, CURC-loaded nanoparticles (NPs) were produced by a double emulsion technique. In particular, NPs were made up of an amphiphilic blend of poloxamers and PLGA to confer stealth properties to the NPs to take advantage of the enhanced permeability and retention (EPR) effect. Different surface properties of NPs made up of bare PLGA and PLGA/poloxamer blend were confirmed by the different interactions of these NPs with serum proteins and also by their ability to be internalized by mesothelioma cell line. The uptake of PLGA/poloxamer NPs induces a persistent block in G0/G1 phase of the cell cycle up to 72 h, thus overcoming the drug tolerance phenomenon, normally evidenced with free CURC.Copyright © 2015 Elsevier B.V. All rights reserved.