Biochemical and Biophysical Research Communications 1993-10-15

A novel peptide from funnel web spider venom, omega-Aga-TK, selectively blocks, P-type calcium channels.

T Teramoto, M Kuwada, T Niidome, K Sawada, Y Nishizawa, K Katayama

Index: Biochem. Biophys. Res. Commun. 196 , 134-140, (1993)

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Abstract

In the course of purification of omega-Aga-IVA, a specific P-type calcium channel blocker, from the venom of Agelenopsis aperta we discovered a novel peptide. This peptide, named omega-agatoxin Tsukuba (omega-Aga-TK), also blocked P-type channels and was twelve times more abundant in the venom than omega-Aga-IVA. omega-Aga-TK was purified to homogeneity by a two-step reverse-phase HPLC procedure. Its amino acid sequence is 71% identical to that of omega-Aga-IVA. omega-Aga-TK has a negatively charged N-terminus, whereas omega-Aga-IVA has a positively charged one. Electrophysiological data indicate that omega-Aga-TK is a potent and selective inhibitor of P-type channels.