FEBS Letters 2014-12-20

Calcium release-activated calcium (CRAC) channels mediate the β(2)-adrenergic regulation of Na,K-ATPase.

Michael J Keller, Emilia Lecuona, Murali Prakriya, Yuan Cheng, Saul Soberanes, G R Scott Budinger, Jacob I Sznajder

Index: FEBS Lett. 588(24) , 4686-93, (2014)

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Abstract

β2-Adrenergic agonists have been shown to regulate Na,K-ATPase in the alveolar epithelium by recruiting Na,K-ATPase-containing vesicles to the plasma membrane of alveolar epithelial cells (AEC). Here, we provide evidence that β2-agonists induce store-operated calcium entry (SOCE) in AECs. This calcium entry is necessary for β2-agonist-induced recruitment of Na,K-ATPase to the plasma membrane of AECs. Specifically, we show that β2-agonists induce SOCE via stromal interaction molecule 1 (STIM1)-associated calcium release-activated calcium (CRAC) channels. We also demonstrate that the magnitude of SOCE affects the abundance of Na,K-ATPase at the plasma membrane of AECs. Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.