International journal of clinical and experimental medicine 2015-01-01

Quantitation of nuclear factor kappa B activation in pancreatic acinar cells during rat acute pancreatitis by flow cytometry.

Wenliang Chen, Zhuanzhen Zheng, Junfang Duan, Xiaoru Wang, Shirong Wu, Wei Wang, Lu Xu, Shuguang Han, Zhenhua Qiao

Index: Int. J. Clin. Exp. Med. 8 , 10143-51, (2015)

Full Text: HTML

Abstract

This study aimed to develop a specific and sensitive method for the rapid detection of NF-κB activity in pancreatic tissue. Male Wistar rats were randomly divided into two groups: (1) 16 rats in the acute pancreatitis (AP) group received retrograde injection of 5% sodium taurocholate (STC) into the biliopancreatic duct, and (2) 16 rats in the Control group received saline. NF-κB activation in rat pancreatic acinar cells was assessed by flow cytometry (FCM). We found that the NF-κB activity in the AP group significantly increased at 1.5 h (29.80%±7.83), had a peak at 3 h (65.17%±13.22), and then decreased gradually to 12 h time point, close to the level after 1.5 h stimulation of STC. The NF-κB activity of the Control group did not significantly vary at different time points (P>0.05). FCM is a specific and sensitive assay for the rapid detection of NF-κB activity in pancreatic tissue.