Fluorescein isothiocyanate
Fluorescein isothiocyanate structure
|
Common Name | Fluorescein isothiocyanate | ||
|---|---|---|---|---|
| CAS Number | 3326-32-7 | Molecular Weight | 389.381 | |
| Density | 1.5±0.1 g/cm3 | Boiling Point | 708.6±60.0 °C at 760 mmHg | |
| Molecular Formula | C21H11NO5S | Melting Point | >360 °C(lit.) | |
| MSDS | Chinese USA | Flash Point | 382.4±32.9 °C | |
| Symbol |
GHS08 |
Signal Word | Danger | |
Use of Fluorescein isothiocyanateFITC is a derivative of fluorescein for the labeling of amines. |
This table lists Chinese names, IUPAC names and various aliases of this chemical substance.
| Name | fluorescein 5-isothiocyanate |
|---|---|
| Synonym | More Synonyms |
This table contains bioactivity, target information and biological‑assay experimental data of this compound.
| Description | FITC is a derivative of fluorescein for the labeling of amines. |
|---|---|
| Related Catalog | |
| In Vitro | Annexin V-FITC/PI is employed to detect chondrocyte apoptosis[1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites[2]. |
| Cell Assay | The frequency of chondrocyte apoptosis is measured by flow cytometry with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide. A total of 1×104 treated chondrocytes are collected from each group, washed in cold PBS and incubated with Annexin V-FITC and PI at room temperature for 15 min in the dark on ice. These samples are then analyzed using a fluorescence-activated cell sorter. Cell Quest software is used to analyze the percentage of apoptosis. All tests are repeated in triplicate. |
| References |
This table shows physicochemical parameters including melting point, boiling point, density, solubility and optical rotation. Missing data is marked as N/A.
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 708.6±60.0 °C at 760 mmHg |
| Melting Point | >360 °C(lit.) |
| Molecular Formula | C21H11NO5S |
| Molecular Weight | 389.381 |
| Flash Point | 382.4±32.9 °C |
| Exact Mass | 389.035797 |
| PSA | 120.44000 |
| LogP | 4.00 |
| Vapour Pressure | 0.0±2.3 mmHg at 25°C |
| Index of Refraction | 1.754 |
| InChIKey | MHMNJMPURVTYEJ-UHFFFAOYSA-N |
| SMILES | O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21 |
| Storage condition | 2-8°C |
| Stability | Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids. |
| Water Solubility | practically insoluble |
This table provides MSDS information including hazard classification, first‑aid, fire‑fighting, spill handling, operation and storage instructions.
This table covers safety warnings, protective measures, incompatible substances and disposal guidelines for this compound.
| Symbol |
GHS08 |
|---|---|
| Signal Word | Danger |
| Hazard Statements | H317-H334 |
| Precautionary Statements | P261-P280-P342 + P311 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Faceshields;Gloves |
| Hazard Codes | Xn: Harmful; |
| Risk Phrases | R22;R36/37/38;R42/43 |
| Safety Phrases | S22-S45-S36/37/39-S26-S36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
This table presents publicly reported synthetic routes, reaction conditions, reactants and product information of the compound.
|
~%
Fluorescein iso... CAS#:3326-32-7 |
| Literature: WO2006/84339 A1, ; Page/Page column 30 ; |
|
~%
Fluorescein iso... CAS#:3326-32-7 |
| Literature: Tomasch, Miriam; Schwed, J. Stephan; Kuczka, Karina; Meyer Dos Santos, Sascha; Harder, Sebastian; Nuesing, Rolf M.; Paulke, Alexander; Stark, Holger ACS Medicinal Chemistry Letters, 2012 , vol. 3, # 9 p. 774 - 779 |
|
~%
Fluorescein iso... CAS#:3326-32-7 |
| Literature: ACS Medicinal Chemistry Letters, , vol. 3, # 9 p. 774 - 779 |
|
~%
Fluorescein iso... CAS#:3326-32-7 |
| Literature: ACS Medicinal Chemistry Letters, , vol. 3, # 9 p. 774 - 779 |
|
~%
Fluorescein iso... CAS#:3326-32-7 |
| Literature: ACS Medicinal Chemistry Letters, , vol. 3, # 9 p. 774 - 779 |
|
~%
Fluorescein iso... CAS#:3326-32-7 |
| Literature: ACS Medicinal Chemistry Letters, , vol. 3, # 9 p. 774 - 779 |
![(6'-acetyloxy-5-nitro-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate structure](https://image.chemsrc.com/caspic/238/14926-29-5.png)
This table lists upstream starting materials and downstream derivative products related to this chemical.
| Precursor 7 | |
|---|---|
| DownStream 0 | |
This table lists relevant public references with title, journal and publication metadata for this compound.
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Imaging of a clinically relevant stroke model: glucose hypermetabolism revisited.
Stroke 46(3) , 835-42, (2015) Ischemic stroke has been shown to cause hypermetabolism of glucose in the ischemic penumbra. Experimental and clinical data indicate that infarct-related systemic hyperglycemia is a potential therapeu... |
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Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties.
Int. J. Nanomedicine 9 , 2815-32, (2014) Silica (SiO₂) nanoparticles (NPs) are widely used in diverse industrial and biomedical applications. Their applicability depends on surface modifications, which can limit potential health problems.To ... |
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Uptake of neutrophil-derived Ym1 protein distinguishes wound macrophages in the absence of interleukin-4 signaling in murine wound healing.
Am. J. Pathol. 184(12) , 3249-61, (2014) The determination of regenerative wound-healing macrophages as alternatively activated macrophages is currently questioned by the absence of IL-4 in wound tissue. Yet, murine wound tissue expressed hi... |
This table contains target information, in‑vitro & in‑vivo assay data for this compound.
This table collects all English aliases, systematic names and CAS‑related synonyms of the compound.
| 5-FITC |
| Isothiocyanatofluorescein |
| 5-Isothionatofluorescein |
| FITC Fluorescein 5-isothiocyanate |
| EINECS 248-207-7 |
| Fluorescein Isothiosyanate |
| fluorescein isothiocyanate isomer 1 |
| fluorescein 6-isothiocyanate |
| FITC ISOMER |
| Fluorescein 5-Isothiocyanate (isomer I) |
| 3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofurane-1,9'-xanthén]-3-one |
| 5-FITC (isomer I) |
| 5-Isothiocyanatofluorescein (isomer I) |
| FITC |
| 5-Fluorescein Isothiocyanate |
| fluorescein 5-isothiocyanate |
| FITC,Fluorescein 5-isothiocyanate |
| FITC isomer I |
| Fluorescein isothiocyanate,isomer 1 |
| 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-isothiocyanatobenzoic acid |
| Fluorescein Isothiocyanate (isomer I) |
| Fluorescein isothiocyanate isomer I |
| FITC-I |
| Fluorescein 5(6)-isothiocyanate |
| Fluorescein-5-isothiocyanate |
| MFCD00005063 |
| Fluorescein Isothiocyanate |
| 3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one |
This section contains frequently‑asked‑questions about this compound, including basic parameters, properties, storage conditions and corresponding answers.
| Q: What is the CAS number of fluorescein 5-isothiocyanate? |
| A: CAS number of fluorescein 5-isothiocyanate is 3326-32-7. |
| Q: What is the polar surface area (PSA) of fluorescein 5-isothiocyanate? |
| A: Polar surface area (PSA) of fluorescein 5-isothiocyanate is 120.44000. |
| Q: What is the melting point of fluorescein 5-isothiocyanate? |
| A: Melting point of fluorescein 5-isothiocyanate is >360 °C(lit.). |
| Q: What are the upstream materials of fluorescein 5-isothiocyanate? |
| A: Related upstream materials(CAS) of fluorescein 5-isothiocyanate: 2321-07-5;463-71-8;3326-34-9;610-27-5;108-46-3;3326-35-0;14926-29-5. |
| Q: What is the SMILES notation of fluorescein 5-isothiocyanate? |
| A: SMILES notation of fluorescein 5-isothiocyanate is O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21. |
| Q: What is the LogP of fluorescein 5-isothiocyanate? |
| A: LogP of fluorescein 5-isothiocyanate is 4.00. |
| Q: What is the molecular weight of fluorescein 5-isothiocyanate? |
| A: Molecular weight of fluorescein 5-isothiocyanate is 389.381. |
| Q: What is the InChIKey of fluorescein 5-isothiocyanate? |
| A: InChIKey of fluorescein 5-isothiocyanate is MHMNJMPURVTYEJ-UHFFFAOYSA-N. |
| Q: What are the uses of fluorescein 5-isothiocyanate? |
| A: Main uses of fluorescein 5-isothiocyanate: FITC is a derivative of fluorescein for the labeling of amines. |
| Q: What is the safety information for fluorescein 5-isothiocyanate? |
| A: Safety information for fluorescein 5-isothiocyanate: S22-S45-S36/37/39-S26-S36/37. |
This table lists manufacturers and suppliers of this compound, including vendor names and product supply reference information.
| Shanghai Nianxing Industrial Co., Ltd |
| Dayang Chem (Hangzhou) Co., Ltd. |