Organic & Biomolecular Chemistry 2012-12-07

Rational substrate and enzyme engineering of transketolase for aromatics.

Panwajee Payongsri, David Steadman, John Strafford, Andrew MacMurray, Helen C Hailes, Paul A Dalby

Index: Org. Biomol. Chem. 10(45) , 9021-9, (2012)

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Abstract

The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.