Journal of Agricultural and Food Chemistry 1999-11-01

Analysis of vervain flavonoids by HPLC/Diode array detector method. Its application to quality control.

P Valentão, P B Andrade, F Areias, F Ferreres, R M Seabra

Index: J. Agric. Food Chem. 47(11) , 4579-82, (1999)

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Abstract

A reversed-phase HPLC procedure is proposed for the determination of seven flavonoids (luteolin, nepetin, hispidulin, jaceosidin, cirsimaritin, cirsilineol, and eupatorin) in vervain samples. A simple extractive technique was developed, involving only extraction with ethyl ether, which allowed the elimination of phenolic acids, yielding a sufficiently purified flavonoid fraction. All of the analyzed vervain samples showed a common flavonoid pattern, in which hispidulin and jaceosidin were the major compounds and nepetin was the minor compound.