Clinica Chimica Acta 1986-06-15

Enzyme electrode for the measurement of lactate in undiluted blood.

W H Mullen, S J Churchouse, F H Keedy, P M Vadgama

Index: Clin. Chim. Acta 157(2) , 191-8, (1986)

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Abstract

An enzyme electrode is described based upon the enzyme lactate oxidase (EC 1.1.3.2) coupled to an H2O2 sensor. Use of an organosilane-treated microporous membrane over the enzyme layer, allows responses linear to 18 mmol/l L-lactate with response times of 1-3 min, whilst giving independence from stirring. The method is suitable for the bedside analysis of a single drop of undiluted whole blood. Correlation with a routine method gave y = 0.96x + 0.11, r = 0.996, n = 46.