Bioorganic & Medicinal Chemistry Letters 2008-04-01

Rhodamine-based fluorogenic probe for imaging biological thiol.

Aya Shibata, Kazuhiro Furukawa, Hiroshi Abe, Satoshi Tsuneda, Yoshihiro Ito

Index: Bioorg. Med. Chem. Lett. 18(7) , 2246-9, (2008)

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Abstract

We have developed a new fluorescent probe for biological thiol. The probe was synthesized by the modification of the 2,4-dinitrobenzenesulfonyl group with rhodamine 110. The selective detection of thiol species such as cysteine or glutathione was achieved in biological conditions. Moreover, the probe was successfully applied to the imaging of thiol species in living human cells.