Rhodamine 110 structure
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Common Name | Rhodamine 110 | ||
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| CAS Number | 13558-31-1 | Molecular Weight | 366.798 | |
| Density | N/A | Boiling Point | 629.3ºC at 760 mmHg | |
| Molecular Formula | C20H15ClN2O3 | Melting Point | >300 °C(lit.) | |
| MSDS | Chinese USA | Flash Point | 334.4ºC | |
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Rhodamine-based fluorogenic probe for imaging biological thiol.
Bioorg. Med. Chem. Lett. 18(7) , 2246-9, (2008) We have developed a new fluorescent probe for biological thiol. The probe was synthesized by the modification of the 2,4-dinitrobenzenesulfonyl group with rhodamine 110. The selective detection of thiol species such as cysteine or glutathione was achieved in ... |
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Early detection of apoptosis in living cells by fluorescence correlation spectroscopy.
Anal. Bioanal. Chem 396(3) , 1177-85, (2010) Early detection of apoptotic cells via caspase activity is demonstrated with fast response time. Fluorescence correlation spectroscopy (FCS) is used to identify the presence of a cleaved fluorogenic probe based on the fluorescence of rhodamine 110 in Jurkat c... |
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Mapping vortex-like hydrodynamic flow in microfluidic networks using fluorescence correlation spectroscopy.
Anal. Chim. Acta 651(1) , 85-90, (2009) The ability to quickly measure flow parameters in microfluidic devices is critical for micro total analysis system (microTAS) applications. Macrofluidic methods to assess flow suffer from limitations that have made conventional methods unsuitable for the flow... |
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Effects of polyoxyethylene (40) stearate on the activity of P-glycoprotein and cytochrome P450.
Eur. J. Pharm. Sci. 37(5) , 573-80, (2009) The present study was aimed to investigate the effects of polyoxyethylene (40) stearate (PS), a non-ionic surfactant, on the activity of P-glycoprotein (P-gp) and six major cytochrome P450 (CYP) isoforms. An in vitro diffusion chamber system was utilized to e... |
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Rhamnolipids enhance epithelial permeability in Caco-2 monolayers.
Int. J. Pharm. 446(1-2) , 130-5, (2013) This work aimed to evaluate the applicability of rhamnolipids as permeation enhancers for oral drugs. In this study, rhamnolipids were found to effectively increase the paracellular and transcellular transport of Transwell(®)-cultured Caco-2 cells, an in vitr... |
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A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells.
Bioorg. Med. Chem. Lett. 18(22) , 5864-6, (2008) A derivative of rhodamine 110 has been designed and assessed as a probe for cytochrome P450 activity. This probe is the first to utilize a 'trimethyl lock' that is triggered by cleavage of an ether bond. In vitro, fluorescence was manifested by the CYP1A1 iso... |
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Quantification of prothrombin in human plasma amplified by autocatalytic reaction.
Anal. Chem. 84(5) , 2380-7, (2012) By site directed mutagenesis, we have produced recombinant mutants of human and mouse prethrombin-2 which are able to convert themselves autocatalytically into α-thrombin. We also have created a new method to amplify the signal of bioanalytical assays based o... |
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Note: a 4 ns hardware photon correlator based on a general-purpose field-programmable gate array development board implemented in a compact setup for fluorescence correlation spectroscopy.
Rev. Sci. Instrum. 83(9) , 096105, (2012) We present a fast hardware photon correlator implemented in a field-programmable gate array (FPGA) combined with a compact confocal fluorescence setup. The correlator has two independent units with a time resolution of 4 ns while utilizing less than 15% of a ... |
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Comparison of methods to classify and quantify free and bound states of complexes using single molecule fluorescence anisotropy.
Analyst 134(9) , 1911-21, (2009) Steady-state single molecule fluorescence anisotropy (SMFA) is described to quantify free and bound probe molecules from a Biotin-Neutravidin complexation reaction. By formulating a ratio of bound to the total number of molecules sampled (N(b)/N(t) ratio) we ... |
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An improved end-point fluorimetric procedure for the determination of low amounts of trypsin activity in biological samples using rhodamine-110-based substrates.
Appl. Biochem. Biotechnol. 160(1) , 1-8, (2010) A novel end-point fluorimetric procedure based on the use of rhodamine-110-labeled specific substrate was developed to determine trypsin activities in biological samples. We evaluated the ability of trichloroacetic acid and acetic acid to stop the enzymatic r... |