Nucleic Acids Symposium Series 1983-01-01

N4-aminocytidine: formation, reactivity, and mutagenicity.

K Negishi, N Nitta, Y Yamashita, M Takahashi, C Harada, K Oohara, M Nishizawa, Y Wataya, H Hayatsu

Index: Nucleic Acids Symp. Ser. (12) , 29-30, (1983)

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Abstract

N4-Aminocytidine was readily formed on treatment of cytidine with hydrazine-bisulfite under mild conditions. This reaction can be carried out for cytosines in polynucleotids as well. The nucleoside N4-aminocytidine was strongly mutagenic to bacteria and phage. The transformation of cytosines in polynucleotides into N4-aminocytosines is a convenient method for generating reactive groups in the polymer.