Antimicrobial Agents and Chemotherapy 2013-03-01

Function of cytochrome P450 enzymes RosC and RosD in the biosynthesis of rosamicin macrolide antibiotic produced by Micromonospora rosaria.

Yohei Iizaka, Noriko Higashi, Masanari Ishida, Reina Oiwa, Yumi Ichikawa, Moeka Takeda, Yojiro Anzai, Fumio Kato

Index: Antimicrob. Agents Chemother. 57(3) , 1529-31, (2013)

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Abstract

The cytochrome P450 enzyme-encoding genes rosC and rosD were cloned from the rosamicin biosynthetic gene cluster of Micromonospora rosaria IFO13697. The functions of RosC and RosD were demonstrated by gene disruption and complementation with M. rosaria and bioconversion of rosamicin biosynthetic intermediates with Escherichia coli expressing RosC and RosD. It is proposed that M. rosaria IFO13697 has two pathway branches that lead from the first desosaminyl rosamicin intermediate, 20-deoxo-20-dihydro-12,13-deepoxyrosamicin, to rosamicin.