European Journal of Biochemistry 1982-03-01

Two-dimensional NMR spectroscopy of siomycin A. Proton--carbon-13 chemical shift correlation.

N J Clayden, F Inagaki, R J Williams, G A Morris, K Tori, K Tokura, T Miyazawa

Index: Eur. J. Biochem. 123(1) , 127-31, (1982)

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Abstract

A trial application of a recent two-dimensional nuclear magnetic resonance experiment to the polypeptide antibiotic siomycin A is described. Proton--carbon-13 chemical shift correlation measures the proton and carbon-13 chemical shift for each directly bonded CH group in a molecule, in a single experiment. The resultant map of correlated chemical shifts enables the carbon-13 spectrum to be assigned directly from the known proton shifts, and allows individual proton signals to be identified without problems of overlap. The signal-to-noise ratio available from such techniques should enable their application to aqueous protein solutions using currently available high-field spectrometers.