Bioscience, Biotechnology, and Biochemistry 2012-01-01

Toxoflavin lyase enzyme as a marker for selecting potato plant transformants.

Mi-Sun Kim, HyunSoon Kim, Jae Sun Moon, Ingyu Hwang, Hyouk Joung, Jae-Heung Jeon

Index: Biosci. Biotechnol. Biochem. 76(12) , 2354-6, (2012)

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Abstract

This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.