FEBS Letters 2012-04-24

NMR characterization of the interaction between the PUB domain of peptide:N-glycanase and ubiquitin-like domain of HR23

Yukiko Kamiya, Yoshinori Uekusa, Akira Sumiyoshi, Hiroaki Sasakawa, Takeshi Hirao, Tadashi Suzuki, Koichi Kato

Index: FEBS Lett. 586(8) , 1141-6, (2012)

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Abstract

Highlights ► PUB domain of PNGase binds to HR23–UBL more preferentially than ubiquitin chains. ► 3D model of the PUB–UBL complex is presented based on NMR data. ► The PUB–UBL complex is mediated by electrostatic interactions. ► PUB binds the UBL surface involved in intramolecular interaction with HR23–UBA. ► PNGase-PUB could serve as a putative activator of HR23 in ERAD.