Bioorganic & Medicinal Chemistry Letters 2011-12-15

A simple synthesis of APM ([p-(N-acrylamino)-phenyl]mercuric chloride), a useful tool for the analysis of thiolated biomolecules.

V Lemau de Talancé, F Bauer, D Hermand, S P Vincent

Index: Bioorg. Med. Chem. Lett. 21(24) , 7265-7, (2011)

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Abstract

This study describes two novel synthetic procedures to prepare APM, a useful tool for the analysis and the purification of thiolated biomolecules. The methods developed are technically simple and robust and allowed the first full characterization of pure APM. Moreover, the efficacy of APM, as a biochemical tool, was demonstrated by analysis of tRNA thiolation by APM-PAGE.Copyright © 2011 Elsevier Ltd. All rights reserved.