Journal of the American Chemical Society 2003-03-05

A modular cross-linking approach for exploring protein interactions.

Michelle Trester-Zedlitz, Katsuhiko Kamada, Stephen K Burley, David Fenyö, Brian T Chait, Tom W Muir

Index: J. Am. Chem. Soc. 125 , 2416 - 2425, (2003)

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Abstract

A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.