Chemical Communications 2005-05-28

Stereoselective hydroxylation of an achiral cyclopentanecarboxylic acid derivative using engineered P450s BM-3.

Dieter F Münzer, Peter Meinhold, Matthew W Peters, Sabine Feichtenhofer, Herfried Griengl, Frances H Arnold, Anton Glieder, Anna de Raadt

Index: Chem. Commun. (Camb.) , 2597, (2005)

Full Text: HTML

Abstract

Substrate engineered, achiral carboxylic acid derivative was biohydroxylated with various mutants of cytochrome P450 BM-3 to give two out of the four possible diastereoisomers in high de and ee. The BM-3 mutants exhibit up to 9200 total turnovers for hydroxylation of the engineered substrate, which without the protecting group is not transformed by this enzyme.