Diagnostic Molecular Pathology 2010-06-01

Successful pyrosequencing of GC-rich DNA sequences by partial substitution of deoxyguanosine with deoxyinosine.

Lulette Tricia C Bravo, Marion J Tuohy, Nabin K Shrestha

Index: Diagn. Mol. Pathol. 19 , 123-125, (2010)

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Abstract

We experienced significant problems while developing a PyrosequencingTM (Biotage, Uppsala, Sweden) assay to characterize the rifampin resistance-determining region of Mycobacteriumtuberculosis, a target with high guanosine-cytosine content. This paper describes the successful use of a modified pyrosequencing protocol through partial substitution of deoxyguanosine triphosphate with deoxyinosine triphosphate in the polymerase chain reaction.