Antimicrobial Agents and Chemotherapy 2009-04-01

Mutations in three distinct loci cause resistance to peptide deformylase inhibitors in Bacillus subtilis.

Yann Duroc, Carmela Giglione, Thierry Meinnel

Index: Antimicrob. Agents Chemother. 53(4) , 1673-8, (2009)

Full Text: HTML

Abstract

Bacillus subtilis mutants with resistance against peptide deformylase inhibitors were isolated. All showed a bypass of the pathway through mutations in three genes required for formylation of Met-tRNA(fMet), fmt, folD, and glyA. glyA corresponds to a yet uncharacterized locus inducing resistance. The bypass of formylation caused robust fitness reduction but was not accompanied by alterations of the transcription profile. A subtle adaptation of the enzymes of the intermediary metabolism was observed.