Journal of the American Society for Mass Spectrometry 2013-06-01

Gas-phase fragmentation of oligoproline peptide ions lacking easily mobilizable protons.

Magdalena Rudowska, Robert Wieczorek, Alicja Kluczyk, Piotr Stefanowicz, Zbigniew Szewczuk

Index: J. Am. Soc. Mass Spectrom. 24 , 846-56, (2013)

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Abstract

The fragmentation of peptides containing quaternary ammonium group, but lacking easily mobilizable protons, was examined with the aid of deuterium-labeled analogs and quantum-chemical modeling. The fragmentation of oligoproline containing quaternary ammonium group involves the mobilization of hydrogens localized at α- and γ- or δ-carbon atoms in the pyrrolidine ring of proline. The study of the dissociation pattern highlights the unusual proline residue behavior during MS/MS experiments of peptides.