Journal of Microbiological Methods 1999-08-01

An improved method for screening alpha-acetolactate producing mutants.

C Monnet, S Haddad, G Corrieu

Index: J. Microbiol. Methods 37(2) , 183-5, (1999)

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Abstract

Alpha-Acetolactate-deficient Lactococcus lactis ssp. lactis biovar. diacelylactis are utilised in several industrial processes for producing diacetyl and alpha-acetolactate. They can be selected by screening after random mutagenesis. We improved a previously described screening method [Monnet, C., Schmitt, P., Diviès, C., 1997. Appl. Environ. Microbiol. 63, 793-795], which makes it possible to screen up to 1000 colonies per agar plate, whereas the previous method allowed to screen only 60 colonies per agar plate. The new screening method facilitates selection of alpha-acetolactate-deficient mutants.