Nucleosides & nucleotides 1998-01-01

Use of 5-nitroindole-2'-deoxyribose-5'-triphosphate for labelling and detection of oligonucleotides.

C L Smith, A C Simmonds, A L Hamilton, D L Martin, A G Lashford, D Loakes, F Hill, D M Brown

Index: Nucleosides Nucleotides 17(1-3) , 555-64, (1998)

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Abstract

The 5'-triphosphate of 5-nitroindole-2'-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole tailed DNA both in single-stranded form and after hybridisation to a template. This is therefore a new method for the detection of nucleic acid probes.