Chemical & Pharmaceutical Bulletin 2000-08-01

Chemiluminescence derivatization of methylglyoxal using 2-aminonicotinic acid.

Y Ohba, M Sakata, K Zaitsu

Index: Chem. Pharm. Bull. 48(8) , 1236-8, (2000)

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Abstract

To develop a sensitive and selective chemiluminometric method for the determination of methylglyoxal, we used 2-aminonicotinic acid as the chemiluminescence derivatization reagent. 2-Aminonicotinic acid reacts with methylglyoxal in an acidic solution at 37 degrees C for 4 h and gave a chemiluminescence in N,N'-dimethylformamide containing sodium tert-butoxide. The detection limit (blank intensity plus three-times its standard deviation of methylglyoxal) for methylglyoxal is 233 fmol in the reaction mixture.