M F Lopez, W F Patton, W B Sawlivich, H Erdjument-Bromage, P Barry, K Gmyrek, T Hines, P Tempst, W M Skea
Index: Biochim. Biophys. Acta 1205(1) , 29-38, (1994)
Full Text: HTML
A novel glutathione S-transferase (GST) was purified from broccoli (Brassica oleracea var. italica). Partial amino-acid sequencing indicated that the protein shared significant homology with several different plant GSTs from maize, silene, Dianthus, Nicotiana and Triticum, but little homology to yeast (Issatchenkia) GST. One region of the polypeptide near the N-terminal also shared significant homology to a region of rat 5-5, rat 12-12 and human theta-GST (collectively referred to as the theta-GST-class) but little structural homology to the common mammalian cytosolic GSTs (alpha-, mu- or pi-classes). The broccoli GST was retained on a novel membrane based glutathione affinity matrix and displayed activity towards 1-chloro-2,4-dinitro-benzene (CDNB), a general GST substrate, as well as 4-nitrophenethyl bromide, a marker substrate for the theta-class of GSTs. The characteristics of the broccoli GST potentially define it as a member of the theta-class. This is consistent with the view that the theta-class may have arisen prior to the divergence of animals and plants while the mammalian mu-, pi- and alpha-classes evolved after the two kingdoms were established.
Structure | Name/CAS No. | Molecular Formula | Articles |
---|---|---|---|
![]() |
1-(2-Bromoethyl)-4-nitrobenzene
CAS:5339-26-4 |
C8H8BrNO2 |
An ensemble of theta class glutathione transferases with nov...
2002-04-19 [J. Mol. Biol. 318(1) , 59-70, (2002)] |
Residue 234 in glutathione transferase T1-1 plays a pivotal ...
2005-05-15 [Biochem. J. 388(Pt 1) , 387-92, (2005)] |
Kinetic characterization of recombinant human glutathione tr...
1997-12-15 [Arch. Biochem. Biophys. 348(2) , 247-54, (1997)] |
Enzymatic inactivation of major circulating forms of atrial ...
1999-04-16 [Eur. J. Pharmacol. 370(3) , 307-12, (1999)] |
Molecular evolution of Theta-class glutathione transferase f...
2010-05-01 [Arch. Biochem. Biophys. 497(1-2) , 28-34, (2010)] |
Home | MSDS/SDS Database Search | Journals | Product Classification | Biologically Active Compounds | Selling Leads | About Us | Disclaimer
Copyright © 2024 ChemSrc All Rights Reserved