Journal of the American Chemical Society

Mechanism of the oxidation of NADH by quinones. Energetics of one-electron and hydride routes

BW Carlson, LL Miller

Index: Carlson, Brian W.; Miller, Larry L. Journal of the American Chemical Society, 1985 , vol. 107, # 2 p. 479 - 485

Full Text: HTML

Citation Number: 186

Abstract

Abstract: The kinetics of NADH oxidation by 7 o-benzoquinones and 14 p-benzoquinones were studied by using buffered aqueous solutions and UV/vis spectroscopy. For each quinone the rate law was first order in NADH and first order in quinone. The rate constants varied from 0.0745 to 9220 M-'s-'. Variation of the pH from 6 to 8 gave no change in rate. The use of 4-D-and 4, 4-D2-NADH revealed kinetic isotope effects. The dideuterio data gave ...