874374-25-1

874374-25-1 structure

Name: Ned 19
Chemical Name: 1-(3-{[4-(2-Fluorophenyl)-1-piperazinyl]methyl}-4-methoxyphenyl)- 2,3,4,9-tetrahydro-1H-β-carboline-3-carboxylic acid
CAS Number: 874374-25-1
Molecular Formula: C₃₀H₃₁FN₄O₃
Molecular Weight: 514.59100
Catalog: Signaling Pathways Membrane Transporter/Ion Channel Calcium Channel
Create Date: 2016-01-31 08:49:47
Modify Date: 2025-08-25 19:05:25
Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with an IC50 of 65 nM[1]. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice[2].

Name	1-(3-{[4-(2-Fluorophenyl)-1-piperazinyl]methyl}-4-methoxyphenyl)- 2,3,4,9-tetrahydro-1H-β-carboline-3-carboxylic acid

Description	Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with an IC50 of 65 nM[1]. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice[2].
Related Catalog	Signaling Pathways >> Membrane Transporter/Ion Channel >> Calcium Channel Research Areas >> Cancer
Target	Ca2+
In Vitro	Ned 19 (25-100 μM; 24-72 hours) reduces cell proliferation[2]. Ned 19 (25-100 μM; 24-72 hours) reduces markedly the cell number[2]. Ned 19 (25-100 μM; 24-72 hours) reduces the S phase percentage and increases of the G0/G1 phase percentage evaluated by cell cycle analysis[2]. Ned 19 (25-100 μM; 24-72 hours) induces cell apoptosis a time-dependent manner[2]. Ned 19 (25-100 μM; 24-72 hours) reduces expression of N-cadherin and increases expression of E-cadherin, affecting the cell migratory behavior[2]. Cell Proliferation Assay[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Reduced cell proliferation. Cell Viability Assay[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Reduced markedly the cell number. Cell Cycle Analysis[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Reduced the S phase percentage and increased of the G0/G1 phase percentage. Apoptosis Analysis[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Induced cell apoptosis a time-dependent manner. Western Blot Analysis[2] Cell Line: B16 cells Concentration: 25, 50, 100 μM Incubation Time: 24, 48, 72 hours Result: Reduced expression of N-cadherin and increased expression of E-cadherin.
In Vivo	Ned 19 (i.p.; 5 mg/Kg/every second day; 4 week) impaires severely tumor growth[2]. Animal Model: Adult male C57BL/6 mice[2] Dosage: 5 mg/Kg Administration: I.p.; every second day; 4 week Result: Impaired severely tumor growth.
References	[1]. Rosen D, et al. Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonistNed-19 indicate two binding sites on the NAADP receptor. J Biol Chem. 2009 Dec 11;284(50):34930-4. [2]. Annarita Favia, et al. NAADP-Dependent Ca2+ Signaling Controls Melanoma Progression, Metastatic Dissemination and Neoangiogenesis. Sci Rep. 2016; 6: 18925.

Molecular Formula	C₃₀H₃₁FN₄O₃
Molecular Weight	514.59100
Exact Mass	514.23800
PSA	80.83000
LogP	4.65780

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